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primary antibodies against myosin heavy chain isoforms  (Developmental Studies Hybridoma Bank)


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    Developmental Studies Hybridoma Bank primary antibodies against myosin heavy chain isoforms
    Baseline morphological and physiological characteristics of the mice following HRW intake. (A) Body weight (g) of the mice consuming purified water (PW) or hydrogen-rich water (HRW) (PW: n = 12 per group, HRW: n = 14 per group). (B) Forelimb grip strength (g/g), measured weekly during the intervention period (PW: n = 12 per group, HRW: n = 14 per group). (C) Wet masses of six hindlimb skeletal muscles (soleus, extensor digitorum longus (EDL), plantaris, tibialis cranialis (TC), gastrocnemius, and quadriceps femoris) (PW: n = 12 per group, HRW: n = 14 per group). (D) Representative images of gastrocnemius muscle cross-sections that were hematoxylin and eosin (HE)-stained or fluorescence immunostained (IF) for myosin heavy <t>chain</t> <t>isoforms</t> ( n = 3 per group).
    Primary Antibodies Against Myosin Heavy Chain Isoforms, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 96/100, based on 53 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primary+antibodies+against+myosin+heavy+chain+isoforms/anti-Myosin+all+isoforms/pmc12864122-60-0-25
    Average 96 stars, based on 53 article reviews
    primary antibodies against myosin heavy chain isoforms - by Bioz Stars, 2026-09
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    1) Product Images from "Hydrogen-rich water improves endurance by reducing skeletal muscle oxidative stress and inflammatory responses"

    Article Title: Hydrogen-rich water improves endurance by reducing skeletal muscle oxidative stress and inflammatory responses

    Journal: Frontiers in Nutrition

    doi: 10.3389/fnut.2026.1722091

    Baseline morphological and physiological characteristics of the mice following HRW intake. (A) Body weight (g) of the mice consuming purified water (PW) or hydrogen-rich water (HRW) (PW: n = 12 per group, HRW: n = 14 per group). (B) Forelimb grip strength (g/g), measured weekly during the intervention period (PW: n = 12 per group, HRW: n = 14 per group). (C) Wet masses of six hindlimb skeletal muscles (soleus, extensor digitorum longus (EDL), plantaris, tibialis cranialis (TC), gastrocnemius, and quadriceps femoris) (PW: n = 12 per group, HRW: n = 14 per group). (D) Representative images of gastrocnemius muscle cross-sections that were hematoxylin and eosin (HE)-stained or fluorescence immunostained (IF) for myosin heavy chain isoforms ( n = 3 per group).
    Figure Legend Snippet: Baseline morphological and physiological characteristics of the mice following HRW intake. (A) Body weight (g) of the mice consuming purified water (PW) or hydrogen-rich water (HRW) (PW: n = 12 per group, HRW: n = 14 per group). (B) Forelimb grip strength (g/g), measured weekly during the intervention period (PW: n = 12 per group, HRW: n = 14 per group). (C) Wet masses of six hindlimb skeletal muscles (soleus, extensor digitorum longus (EDL), plantaris, tibialis cranialis (TC), gastrocnemius, and quadriceps femoris) (PW: n = 12 per group, HRW: n = 14 per group). (D) Representative images of gastrocnemius muscle cross-sections that were hematoxylin and eosin (HE)-stained or fluorescence immunostained (IF) for myosin heavy chain isoforms ( n = 3 per group).

    Techniques Used: Purification, Muscles, Staining, Fluorescence

    Related Articles

    Incubation:

    Article Title: Elimination of myotonia improves myopathy in a muscleblind knockout model of myotonic dystrophy
    Article Snippet: Additional blocking with 0.1mg/mL AffiniPure Fab fragment anti–mouse IgG (3%; H&L) (Jackson Immunoresearch) in PBS with 2% normal goat serum for 60-minutes (room temperature). .. Slides were then washed (3 times with fresh PBS for 10-mins at room temperature) and incubated with primary antibodies (Supplemental Table 1b) overnight (14-18 hours) at 4°C; primary antibodies against myosin heavy chain isoforms were obtained from the Developmental Studies Hybridoma Bank (DSHB) created by the NICHD of the NIH and maintained at The University of Iowa, Department of Biology (Iowa City, IA). ..



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    primary antibodies against myosin heavy chain isoforms  (Developmental Studies Hybridoma Bank)
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    Developmental Studies Hybridoma Bank primary antibodies against myosin heavy chain isoforms
    Baseline morphological and physiological characteristics of the mice following HRW intake. (A) Body weight (g) of the mice consuming purified water (PW) or hydrogen-rich water (HRW) (PW: n = 12 per group, HRW: n = 14 per group). (B) Forelimb grip strength (g/g), measured weekly during the intervention period (PW: n = 12 per group, HRW: n = 14 per group). (C) Wet masses of six hindlimb skeletal muscles (soleus, extensor digitorum longus (EDL), plantaris, tibialis cranialis (TC), gastrocnemius, and quadriceps femoris) (PW: n = 12 per group, HRW: n = 14 per group). (D) Representative images of gastrocnemius muscle cross-sections that were hematoxylin and eosin (HE)-stained or fluorescence immunostained (IF) for myosin heavy <t>chain</t> <t>isoforms</t> ( n = 3 per group).
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    Baseline morphological and physiological characteristics of the mice following HRW intake. (A) Body weight (g) of the mice consuming purified water (PW) or hydrogen-rich water (HRW) (PW: n = 12 per group, HRW: n = 14 per group). (B) Forelimb grip strength (g/g), measured weekly during the intervention period (PW: n = 12 per group, HRW: n = 14 per group). (C) Wet masses of six hindlimb skeletal muscles (soleus, extensor digitorum longus (EDL), plantaris, tibialis cranialis (TC), gastrocnemius, and quadriceps femoris) (PW: n = 12 per group, HRW: n = 14 per group). (D) Representative images of gastrocnemius muscle cross-sections that were hematoxylin and eosin (HE)-stained or fluorescence immunostained (IF) for myosin heavy <t>chain</t> <t>isoforms</t> ( n = 3 per group).
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    primary antibody against myosin heavy chain isoforms myhc i  (Developmental Studies Hybridoma Bank)
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    Baseline morphological and physiological characteristics of the mice following HRW intake. (A) Body weight (g) of the mice consuming purified water (PW) or hydrogen-rich water (HRW) (PW: n = 12 per group, HRW: n = 14 per group). (B) Forelimb grip strength (g/g), measured weekly during the intervention period (PW: n = 12 per group, HRW: n = 14 per group). (C) Wet masses of six hindlimb skeletal muscles (soleus, extensor digitorum longus (EDL), plantaris, tibialis cranialis (TC), gastrocnemius, and quadriceps femoris) (PW: n = 12 per group, HRW: n = 14 per group). (D) Representative images of gastrocnemius muscle cross-sections that were hematoxylin and eosin (HE)-stained or fluorescence immunostained (IF) for myosin heavy <t>chain</t> <t>isoforms</t> ( n = 3 per group).
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    Baseline morphological and physiological characteristics of the mice following HRW intake. (A) Body weight (g) of the mice consuming purified water (PW) or hydrogen-rich water (HRW) (PW: n = 12 per group, HRW: n = 14 per group). (B) Forelimb grip strength (g/g), measured weekly during the intervention period (PW: n = 12 per group, HRW: n = 14 per group). (C) Wet masses of six hindlimb skeletal muscles (soleus, extensor digitorum longus (EDL), plantaris, tibialis cranialis (TC), gastrocnemius, and quadriceps femoris) (PW: n = 12 per group, HRW: n = 14 per group). (D) Representative images of gastrocnemius muscle cross-sections that were hematoxylin and eosin (HE)-stained or fluorescence immunostained (IF) for myosin heavy <t>chain</t> <t>isoforms</t> ( n = 3 per group).
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    Baseline morphological and physiological characteristics of the mice following HRW intake. (A) Body weight (g) of the mice consuming purified water (PW) or hydrogen-rich water (HRW) (PW: n = 12 per group, HRW: n = 14 per group). (B) Forelimb grip strength (g/g), measured weekly during the intervention period (PW: n = 12 per group, HRW: n = 14 per group). (C) Wet masses of six hindlimb skeletal muscles (soleus, extensor digitorum longus (EDL), plantaris, tibialis cranialis (TC), gastrocnemius, and quadriceps femoris) (PW: n = 12 per group, HRW: n = 14 per group). (D) Representative images of gastrocnemius muscle cross-sections that were hematoxylin and eosin (HE)-stained or fluorescence immunostained (IF) for myosin heavy <t>chain</t> <t>isoforms</t> ( n = 3 per group).
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    Baseline morphological and physiological characteristics of the mice following HRW intake. (A) Body weight (g) of the mice consuming purified water (PW) or hydrogen-rich water (HRW) (PW: n = 12 per group, HRW: n = 14 per group). (B) Forelimb grip strength (g/g), measured weekly during the intervention period (PW: n = 12 per group, HRW: n = 14 per group). (C) Wet masses of six hindlimb skeletal muscles (soleus, extensor digitorum longus (EDL), plantaris, tibialis cranialis (TC), gastrocnemius, and quadriceps femoris) (PW: n = 12 per group, HRW: n = 14 per group). (D) Representative images of gastrocnemius muscle cross-sections that were hematoxylin and eosin (HE)-stained or fluorescence immunostained (IF) for myosin heavy <t>chain</t> <t>isoforms</t> ( n = 3 per group).
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    primary antibody against developmental isoform of myosin heavy chain  (Novocastra)
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    Healthy (#3H), GRMD (#6G), and GRMD MuStem (#7G Mu ) dog muscles are presented respectively in left, mid and right panel. (A, B, C) Muscle tissue presentation after hemalun eosin safran staining. (B) Mock GRMD dogs display a typical dystrophinopathic pattern with diffuse anisocytosis, hypertrophic hyaline fibres (open arrowhead), centronucleated fibres (arrow), necrotic fibres (*), regenerative foci (black arrowhead) and multifocal thickening of the endomysial space by fibrosis (highlighted by the saffron yellow staining). (C) The dystrophinopathic pattern is remodelled in GRMD MuStem dogs: anisocytosis is milder, hypertrophic hyaline fibres and necrotic fibres are less numerous, whereas centronucleated fibres are more abundant (arrow) compared to GRMD dogs. Inset, another picture taken on the same tissue sample with a higher magnification that displays centronucleated fibre. (D, E, F) Regenerative activity of muscle fibres in healthy, GRMD and GRMD MuStem dogs, as indicated after immunolabelling specific to the developmental <t>isoform</t> of the myosin heavy chain (MyHCd). (G, H, <t>I)</t> <t>Dystrophin</t> expression in healthy, GRMD and GRMD MuStem dogs. (A-B-C; G-H-I) Scale bar = 200 μm (in set Scale bar = 100 μm). (D, E, F) Scale bar = 500 μm.
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    primary antibodies against myosin heavy chain  (Developmental Studies Hybridoma Bank)
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    Healthy (#3H), GRMD (#6G), and GRMD MuStem (#7G Mu ) dog muscles are presented respectively in left, mid and right panel. (A, B, C) Muscle tissue presentation after hemalun eosin safran staining. (B) Mock GRMD dogs display a typical dystrophinopathic pattern with diffuse anisocytosis, hypertrophic hyaline fibres (open arrowhead), centronucleated fibres (arrow), necrotic fibres (*), regenerative foci (black arrowhead) and multifocal thickening of the endomysial space by fibrosis (highlighted by the saffron yellow staining). (C) The dystrophinopathic pattern is remodelled in GRMD MuStem dogs: anisocytosis is milder, hypertrophic hyaline fibres and necrotic fibres are less numerous, whereas centronucleated fibres are more abundant (arrow) compared to GRMD dogs. Inset, another picture taken on the same tissue sample with a higher magnification that displays centronucleated fibre. (D, E, F) Regenerative activity of muscle fibres in healthy, GRMD and GRMD MuStem dogs, as indicated after immunolabelling specific to the developmental <t>isoform</t> of the myosin heavy chain (MyHCd). (G, H, <t>I)</t> <t>Dystrophin</t> expression in healthy, GRMD and GRMD MuStem dogs. (A-B-C; G-H-I) Scale bar = 200 μm (in set Scale bar = 100 μm). (D, E, F) Scale bar = 500 μm.
    Primary Antibodies Against Myosin Heavy Chain, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Baseline morphological and physiological characteristics of the mice following HRW intake. (A) Body weight (g) of the mice consuming purified water (PW) or hydrogen-rich water (HRW) (PW: n = 12 per group, HRW: n = 14 per group). (B) Forelimb grip strength (g/g), measured weekly during the intervention period (PW: n = 12 per group, HRW: n = 14 per group). (C) Wet masses of six hindlimb skeletal muscles (soleus, extensor digitorum longus (EDL), plantaris, tibialis cranialis (TC), gastrocnemius, and quadriceps femoris) (PW: n = 12 per group, HRW: n = 14 per group). (D) Representative images of gastrocnemius muscle cross-sections that were hematoxylin and eosin (HE)-stained or fluorescence immunostained (IF) for myosin heavy chain isoforms ( n = 3 per group).

    Journal: Frontiers in Nutrition

    Article Title: Hydrogen-rich water improves endurance by reducing skeletal muscle oxidative stress and inflammatory responses

    doi: 10.3389/fnut.2026.1722091

    Figure Lengend Snippet: Baseline morphological and physiological characteristics of the mice following HRW intake. (A) Body weight (g) of the mice consuming purified water (PW) or hydrogen-rich water (HRW) (PW: n = 12 per group, HRW: n = 14 per group). (B) Forelimb grip strength (g/g), measured weekly during the intervention period (PW: n = 12 per group, HRW: n = 14 per group). (C) Wet masses of six hindlimb skeletal muscles (soleus, extensor digitorum longus (EDL), plantaris, tibialis cranialis (TC), gastrocnemius, and quadriceps femoris) (PW: n = 12 per group, HRW: n = 14 per group). (D) Representative images of gastrocnemius muscle cross-sections that were hematoxylin and eosin (HE)-stained or fluorescence immunostained (IF) for myosin heavy chain isoforms ( n = 3 per group).

    Article Snippet: Primary antibodies against myosin heavy chain isoforms were applied overnight at 4 °C (type I (BA-D5), IIa (SC-71), IIx (6H1), or IIb (BF-F3) (all from DSHB, Iowa City, IA, USA)).

    Techniques: Purification, Muscles, Staining, Fluorescence

    Healthy (#3H), GRMD (#6G), and GRMD MuStem (#7G Mu ) dog muscles are presented respectively in left, mid and right panel. (A, B, C) Muscle tissue presentation after hemalun eosin safran staining. (B) Mock GRMD dogs display a typical dystrophinopathic pattern with diffuse anisocytosis, hypertrophic hyaline fibres (open arrowhead), centronucleated fibres (arrow), necrotic fibres (*), regenerative foci (black arrowhead) and multifocal thickening of the endomysial space by fibrosis (highlighted by the saffron yellow staining). (C) The dystrophinopathic pattern is remodelled in GRMD MuStem dogs: anisocytosis is milder, hypertrophic hyaline fibres and necrotic fibres are less numerous, whereas centronucleated fibres are more abundant (arrow) compared to GRMD dogs. Inset, another picture taken on the same tissue sample with a higher magnification that displays centronucleated fibre. (D, E, F) Regenerative activity of muscle fibres in healthy, GRMD and GRMD MuStem dogs, as indicated after immunolabelling specific to the developmental isoform of the myosin heavy chain (MyHCd). (G, H, I) Dystrophin expression in healthy, GRMD and GRMD MuStem dogs. (A-B-C; G-H-I) Scale bar = 200 μm (in set Scale bar = 100 μm). (D, E, F) Scale bar = 500 μm.

    Journal: PLoS ONE

    Article Title: Differential Gene Expression Profiling of Dystrophic Dog Muscle after MuStem Cell Transplantation

    doi: 10.1371/journal.pone.0123336

    Figure Lengend Snippet: Healthy (#3H), GRMD (#6G), and GRMD MuStem (#7G Mu ) dog muscles are presented respectively in left, mid and right panel. (A, B, C) Muscle tissue presentation after hemalun eosin safran staining. (B) Mock GRMD dogs display a typical dystrophinopathic pattern with diffuse anisocytosis, hypertrophic hyaline fibres (open arrowhead), centronucleated fibres (arrow), necrotic fibres (*), regenerative foci (black arrowhead) and multifocal thickening of the endomysial space by fibrosis (highlighted by the saffron yellow staining). (C) The dystrophinopathic pattern is remodelled in GRMD MuStem dogs: anisocytosis is milder, hypertrophic hyaline fibres and necrotic fibres are less numerous, whereas centronucleated fibres are more abundant (arrow) compared to GRMD dogs. Inset, another picture taken on the same tissue sample with a higher magnification that displays centronucleated fibre. (D, E, F) Regenerative activity of muscle fibres in healthy, GRMD and GRMD MuStem dogs, as indicated after immunolabelling specific to the developmental isoform of the myosin heavy chain (MyHCd). (G, H, I) Dystrophin expression in healthy, GRMD and GRMD MuStem dogs. (A-B-C; G-H-I) Scale bar = 200 μm (in set Scale bar = 100 μm). (D, E, F) Scale bar = 500 μm.

    Article Snippet: Sections were incubated (overnight, 4°C) with primary antibody against dystrophin (1:50; NCL-DYS2, Novocastra) or the developmental isoform of myosin heavy chain (MyHCd, 1:100; Novocastra).

    Techniques: Muscles, Staining, Activity Assay, Expressing